I've had three Hondas now - a 1991 NSR125 2stroke bike, a 1998 Honda civic EJ9, and now the 2002 EP3. I have always experimented with different fuels and my conclusion is that yes it does make a difference.
The NSR125 used to hate normal 95oct unleaded and would run noticeably 'rougher' and vibrate through the bars a lot more. Stick some V-power in and the pick-up when coming off roundabouts was much smoother and it felt that little bit stronger in the powerband (6-9.5k rpm).
The EJ9 civic would produce its power between 4k-6.5k rpm, which in effect kind of felt like a powerband in itself (typical small honda engine). The car had all the common breathing mods including a decat, and the tone actually changed slightly when running on V-power. The exhaust just sounded crisper, as if the fuel was burning cleaner. Also because of the decat, town driving could become jerky at times on normal unleaded. When running V-power this problem went away....go figure? Also, fuel consumption was probably boosted by 15 miles or so for every 20ltrs of petrol as you don't really need to use quite as much throttle.
I cant really comment on the EP3 yet as I'm still getting used to the power of the bloody thing. It seems I've finally got a car which you cant regularly use all of the power available simply down to so much slow traffic on the roads. Having said that, the pick-up does seem smoother than when I first picked it up as the previous lady owner just used 95oct fuel. Whether this is just because its not been driven with any anger in a while and its loosened up a little though I'm not sure.
All I know is, now the petrol prices have edged below a quid a litre again I'm just gonna use as much of the stuff as poss while its still affordable. :smt047